● What is the purpose of this exercise?
The purpose of this exercise is to become familiar with the terms and usage of a microscope.
●Are there any safety concerns associated with this exercise? If so, list what they are and what precautions should be taken. Care must be taken while handling the microscope so as not to accidentally drop anything and harm oneself.
A. The following statements are true or false. If true, write a “T” on the answer line. If false, write a word or phrase in the blank to make the statement true.
F | 1) The microscope lens may be cleaned with any soft tissue. | F | 2) The coarse adjustment knob maybe used in focusing with all objective lenses. | T | 3) When beginning to focus, the lowest power lens should be used. | T | 4) Resolution decreases as the amount wavelength of light increases. | F | 5) When focusing always focus toward the specimen. |
B. Match the microscope structures in column B with the statements or phrases about them in column A.
Column A | Column B | Answers |
1) Platform on which the slide rests for viewing | a. nosepiece | 1. C | 2) Used for precise focusing after initial focusing | b. eyepiece | 2. D | 3) Used to increase the amount of light passing through the specimen | c. stage | 3. E | 4) Lens located on the superior end of the body tube | d. fine focus knob | 4. B | 5) Carries the objective lenses and rotates to change magnification | e. iris diaphragm | 5. A |
C. Explain the proper technique for transporting a microscope.
When moving a microscope, one should use two hands and place one hand around the arm of the microscope and the other hand under the base.
D. Define the following terms:
1. field of viewarea of a specimen that can be seen through the microscope
2. resolutionhow well the lens distinguishes between two points; the numerical aperture of the objective lens
3. total magnificationTotal Magnification is the product of the eyepiece power by the objective lens in place.
4. focal lengththe distance that a lens needs to bring light to a focus.
5. contrastthe difference in lighting between two areas of the specimen that are adjacent to each other.
E. What happens to the size of the field of view as magnification increases?The field of view size decreases as magnification increases.
F.What would be the total magnification of a specimen if the eye piece is 15X and the objective lens selected is 4X? 60X
G. If observing a specimen on the low power objective, when switching to high power, the specimen is no longer visible. Why did this happen? The focus might be off of the specimen.
Why is proper microscope technique important for studying Anatomy and Physiology? Since the study of anatomy and physiology is about the structure and function of what makes up the body, there are many microscopic tissues and cells that, if studied through a microscope, will help give a clearer understanding of the subject.