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Effect of temperature on enzyme activity Essay Sample

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Effect of temperature on enzyme activity Essay Sample

I.
Purpose:
Determine how effect of temperature on enzyme (catalyse) activity (in the liver).

II. Materials

* Raw liver

* Forceps

* 50mL 1% hydrogen Peroxide (H

* 25mL graduated cylinder

* 50mL, 400mL beakers

* Pureed liver

* 5 Filter-paper disks

* Paper towels

* Timer or stopwatch

* Thermometer

* Hot plate

III. Procedure

1.Gather all necessary materials; start water bath with 120 mLs of water in the 400 mL beaker. 2.Using the graduated cylinder, pour twenty-five mLs of H2O2 into the 50 mL beaker 3.Gently place the beaker of H2O2 into the water bath; heat the H2O2 to thirty-seven degrees Centigrade, or ninety-eight point six degrees Fahrenheit 4.Once the solution is at the desired temperature, with the forceps, dip a filter-paper disk into the pureed liver and place on a paper towel to remove excess liquid 5.Using the forceps, place the filter-paper at the bottom of the H2O2 solution and release it. 6.Using the stopwatch, keep track of the amount of time it takes for the filter-paper to float to the top of the solution. 7.Record the time it takes for the filter-paper to reach the top of the solution in a data table. 8.Repeat steps four through seven three more times, changing the H2O2 solution when necessary.

IV. Data
Temperature| Time| Observation|
4C| 0| Instantly up|
23C| 5.2| Room temperature|
37C| 0| Floated on the top instantly|
40C| 0| Floated right away|
93.3C| 0| Floated quickly|

V. Result

1. The rate of the reaction was affected by the temperature in such a way that the time it took the reaction to occur significantly decreased. 2. The increase in temperature helped the enzyme react with the liver faster than it did at colder temperatures; The enzyme was most active at the normal temperature of the human body, which is significant because it shows that the enzymes in the body work to their fullest extent when the body temperature is normal, rather than too cold or too hot (hypothermia, hyperthermia). 3. Raw liver was used over cooked liver because in the raw liver, none of the proteins have undergone denaturation, which is the permanent damage or change in the protein that occurs when there is a significant increase or decrease in temperature. Cooking the liver would have caused the proteins to undergo a permanent change, which would have skewed the results of the lab.

4. The human body should maintain a stable internal temperature to not only keep the enzymes working at their best, but also to keep the proteins from undergoing the denaturation process that can cause significant damage in the body, which is usually irreparable. 5. One potential source of error in the procedure of the use of small filter-paper disks. There was such a significantly small amount of liver on these disks that the reactions happened almost instantly, no matter the difference in temperature, making it harder for the students to record accurate results. Use of larger filter-paper disks, or maybe even solid chunks of liver, might have been better to use to gain accurate data.

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