We use cookies to give you the best experience possible. By continuing we’ll assume you’re on board with our cookie policy

Effect of temperature on enzyme activity Essay Sample

essay
  • Pages: 3
  • Word count: 585
  • Rewriting Possibility: 99% (excellent)
  • Category: protein

Get Full Essay

Get access to this section to get all help you need with your essay and educational issues.

Get Access

Introduction of TOPIC

I.
Purpose:
Determine how effect of temperature on enzyme (catalyse) activity (in the liver).

II. Materials

* Raw liver

* Forceps

* 50mL 1% hydrogen Peroxide (H

* 25mL graduated cylinder

* 50mL, 400mL beakers

* Pureed liver

* 5 Filter-paper disks

* Paper towels

* Timer or stopwatch

* Thermometer

* Hot plate

III. Procedure

1.Gather all necessary materials; start water bath with 120 mLs of water in the 400 mL beaker. 2.Using the graduated cylinder, pour twenty-five mLs of H2O2 into the 50 mL beaker 3.Gently place the beaker of H2O2 into the water bath; heat the H2O2 to thirty-seven degrees Centigrade, or ninety-eight point six degrees Fahrenheit 4.Once the solution is at the desired temperature, with the forceps, dip a filter-paper disk into the pureed liver and place on a paper towel to remove excess liquid 5.Using the forceps, place the filter-paper at the bottom of the H2O2 solution and release it. 6.Using the stopwatch, keep track of the amount of time it takes for the filter-paper to float to the top of the solutio

n. 7.Record the time it takes for the filter-paper to reach the top of the solution in a data table.

Sorry, but full essay samples are available only for registered users

Choose a Membership Plan
8.Repeat steps four through seven three more times, changing the H2O2 solution when necessary.

IV. Data
Temperature| Time| Observation|
4C| 0| Instantly up|
23C| 5.2| Room temperature|
37C| 0| Floated on the top instantly|
40C| 0| Floated right away|
93.3C| 0| Floated quickly|

V. Result

1. The rate of the reaction was affected by the temperature in such a way that the time it took the reaction to occur significantly decreased. 2. The increase in temperature helped the enzyme react with the liver faster than it did at colder temperatures; The enzyme was most active at the normal temperature of the human body, which is significant because it shows that the enzymes in the body work to their fullest extent when the body temperature is normal, rather than too cold or too hot (hypothermia, hyperthermia). 3. Raw liver was used over cooked liver because in the raw liver, none of the proteins have undergone denaturation, which is the permanent damage or change in the protein that occurs when there is a significant increase or decrease in temperature. Cooking the liver would have caused the proteins to undergo a permanent change, which would have skewed the results of the lab.

4. The human body should maintain a stable internal temperature to not only keep the enzymes working at their best, but also to keep the proteins from undergoing the denaturation process that can cause significant damage in the body, which is usually irreparable. 5. One potential source of error in the procedure of the use of small filter-paper disks. There was such a significantly small amount of liver on these disks that the reactions happened almost instantly, no matter the difference in temperature, making it harder for the students to record accurate results. Use of larger filter-paper disks, or maybe even solid chunks of liver, might have been better to use to gain accurate data.

We can write a custom essay on

Effect of temperature on enzyme activity Essay Sam ...
According to Your Specific Requirements.

Order an essay

You May Also Find These Documents Helpful

Characterized enolase of A

Here I have characterized enolase of A. fumigatus as a moonlighting protein that binds human Plg. Enolase, in addition to cytosolic location, is expressed on the surface of swollen conidia and hyphae of opportunistic fungal pathogen A. fumigatus. Enolase on the surface of the fungus binds human Plg and the binding is mediated by lysine residues. Furthermore, enolase bound Plg in presence of activator tPa, is functionally active and degrades the chromogenic substrate S2251, the natural substrate fibrinogen and complement proteins C3 and C3b. Moreover, Plg bound to the surface of A. fumigatus in presence of tPa damages lung epithelial cells. In addition, enolase binds complement regulators Factor H, FHL-1 and C4BP, which retain functional activity. Thus, enolase is a moonlighting protein in the surface of the opportunistic fungal pathogen A. fumigatus and enolase binds human Plg, Factor H, FHL-1 and C4BP. The fungus A. fumigatus, in the course of...

Calculating Protein Concentration Using Protein Standard Curve

Quantitative Test for Proteins2Introduction Proteins, a very important macromolecule, have many different function essential in the body. One of the most important role of proteins in the body is to help with muscle building and muscle recovery, which is the reason why protein powders are commonly used in fitness. It is important to know the concentration of protein within an unknown sample to determine how much protein is absorbed in the body and be able to design an effective workout plan along with the proper diet plan in order to achieve desired results in fitness. The manufacturer of a protein powder called “Tough Guy” claims that if a person was to dilute 1 gram of protein powder in 100 ml of water, the final concentration of protein should be approximately 0.40 mg/ml (+/- 5%) protein. A procedure requiring the use of Bio-Rad protein assay will be conducted in order to...

Investigate the Amount of Protein

1. Aim To investigate the amount of protein present in different types of milk by measuring the mass of protein in each types of milk. 2. Introduction Casein is a protein which is found in milk. This is a conjugated protein that has prosthetic groups that is called phosphoric acid, covalently bonded to the R-groups of some amino acid residues. Thus casein is also called phosphoprotein. Casein is insoluble in water. The addition of dilute acid to milk - the precipitate is known as acid casein. In order to estimate the amount of protein present in each type of milk, we can measure the mass of its coagulation in acid. Hypothesis -The mass of protein in full cream milk is highest while the mass of protein in soya milk is lowest. Independent variable Four different types of milk, namely full cream milk, skimmed milk, goat's milk and soya milk. Dependent...

Popular Essays

logo

Emma Taylor

online

Hi there!
Would you like to get such a paper?
How about getting a customized one?