We use cookies to give you the best experience possible. By continuing we’ll assume you’re on board with our cookie policy

Enzyme Catalysis Lab Report

essay
The whole doc is available only for registered users
  • Pages: 2
  • Word count: 380
  • Category: Enzyme

A limited time offer! Get a custom sample essay written according to your requirements urgent 3h delivery guaranteed

Order Now

Background: Enzymes are catalyst, which affect the rate of a chemical reaction. One consequence includes the cell to carry out complex chemical activities at relatively low temperatures. In these reactions the substrate binds reversibly to the active site. The cause of this is a decrease in the energy needed to activate the reaction of the substrate molecule to from products. Every enzyme is particular for a reaction for the reason of its amino acid sequence and unique three-dimensional structure. There are many things that can affect the activity of the enzyme. For example, as a pH is lowered an enzyme will gain H+ ions, and side chains will be affected and the enzymes shape will be disrupted. Since enzymes are catalysts for chemical reactions, enzyme reactions tend to go faster with increasing temperatures. Also if a molecule increases the rate of the reaction it is known as an activator, while if it decreases the rate it is an inhibitor.

The enzyme catalase has four polypeptide chains, each composed of more than 500 amino acids. This enzyme prevents the accumulation of toxic levels of hydrogen peroxide formed as byproducts of metabolic processes. Purpose: The purpose of this lab is to observe an alteration of hydrogen peroxide to water and oxygen gas by the enzyme catalase. This also includes the measurement of the amount of oxygen produced and accumulates the ratio of the enzyme-catalyzed reaction. Hypothesis: I believe with the inclusion of the enzyme catalase the reactions will begin to at a rapid pace but will eventually decrease with the addition of the hydrogen peroxide. Variables: The Concentration of Catalase and the concentration of Hydrogen Peroxide are the dependent variables. The amount of time is the independent variables. The constants include the amounts of each solution and enzyme. Materials & Methods

Materials:
* 10mL of 1.5% H202
* 50mL glass beaker
* 1ml catalase
* 10mL H2SO4
* Burette of KMnO4

Procedures:
a. Put 10 mL of 1.5% H202 in a clean 50 mL glass beaker.
b. Add 1 ml of catalase extract.
c. Swirl gently for 10 seconds.
d. At 10,90,120,180,360 seconds add 10 mL of H2SO4
e. Remove a 5 mL sample and assay for the amount of H2O2 in the sample
f. Use a burette to add KMnO4, drop at a time to the solution.

Related Topics

We can write a custom essay

According to Your Specific Requirements

Order an essay
icon
300+
Materials Daily
icon
100,000+ Subjects
2000+ Topics
icon
Free Plagiarism
Checker
icon
All Materials
are Cataloged Well

Sorry, but copying text is forbidden on this website. If you need this or any other sample, we can send it to you via email.

By clicking "SEND", you agree to our terms of service and privacy policy. We'll occasionally send you account related and promo emails.
Sorry, but only registered users have full access

How about getting this access
immediately?

Your Answer Is Very Helpful For Us
Thank You A Lot!

logo

Emma Taylor

online

Hi there!
Would you like to get such a paper?
How about getting a customized one?

Can't find What you were Looking for?

Get access to our huge, continuously updated knowledge base

The next update will be in:
14 : 59 : 59