We use cookies to give you the best experience possible. By continuing we’ll assume you’re on board with our cookie policy

Introduction to spectrophotometry lab Essay Sample

essay
The whole doc is available only for registered users OPEN DOC
  • Pages:
  • Word count: 228
  • Category: beer light

A limited time offer!

Get a custom sample essay written according to your requirements urgent 3h delivery guaranteed

Order Now

Introduction to spectrophotometry lab Essay Sample

The spectrophotometer is an essential tool for biologists and chemists in analyzing chemical and biological samples. Gaining familiarity with its operating protocols and understanding what its outputs mean are very important in the development of lab technique for students of cell biology. This experiment will help laboratory students gain experience in using the spectrophotometer. Basic Laws of Light Absorption – For a uniform absorbing medium (solution: solvent and solute molecules that absorb light) the proportion of light radiation passing through it is called the transmittance, T, and the proportion of light absorbed by molecules in the medium is absorbance, Abs. Transmittance is defined as:

T = I/Io where: Io= intensity of the incident radiation entering the medium. I = intensity of the transmitted radiation leaving the medium. T is usually expressed as percent transmittance, %T:

%T = I/Io x 100
The Beer-Lambert law (or Beer’s law) is the linear relationship between absorbance and concentration of an absorbing species. The general Beer-Lambert law is usually written as: A = a() * b * c
where A is the measured absorbance, a() is a wavelength-dependent absorptivity coefficient, b is the path length, and c is the analyte concentration. When working in concentration units of molarity, the Beer-Lambert law is written as: A = * b * c
where is the wavelength-dependent molar absorptivity coefficient with units of M-1 cm-1.

We can write a custom essay

According to Your Specific Requirements

Order an essay
Get Access To The Full Essay
icon
300+
Materials Daily
icon
100,000+ Subjects
2000+ Topics
icon
Free Plagiarism
Checker
icon
All Materials
are Cataloged Well

Sorry, but copying text is forbidden on this website. If you need this or any other sample, we can send it to you via email.

By clicking "SEND", you agree to our terms of service and privacy policy. We'll occasionally send you account related and promo emails.
Sorry, but only registered users have full access

How about getting this access
immediately?

Become a member

Your Answer Is Very Helpful For Us
Thank You A Lot!

logo

Emma Taylor

online

Hi there!
Would you like to get such a paper?
How about getting a customized one?

Can't find What you were Looking for?

Get access to our huge, continuously updated knowledge base

The next update will be in:
14 : 59 : 59
Become a Member