We use cookies to give you the best experience possible. By continuing we’ll assume you’re on board with our cookie policy

The Effect of Temperature on Enzymatic Activity Essay Sample

The whole doc is available only for registered users OPEN DOC
  • Pages:
  • Word count: 741
  • Category: enzyme

Get Full Essay

Get access to this section to get all the help you need with your essay and educational goals.

Get Access

The Effect of Temperature on Enzymatic Activity Essay Sample

To investigate enzyme activity of yeast on glucose at different temperatures


As the temperature increases, enzyme activity will also increase. At lower temperatures enzyme activity will be less than when the temperature is higher. Also if temperature is increased too much, enzymatic activity will fall.

This is because the molecules in glucose will move faster and the enzymes will be have increased activity because of increased movement. Also since all enzymes have an optimum temperature at which enzymatic activity is the highest, increasing the temperature will move enzyme closer to optimum temperature. But if temperature is increased too much, enzyme will start to denature (loss of three-dimensional structure) and thus the enzymatic activity will fall again


1. 1. Place 6 ice cubes and 80 mL of water into Beaker A

2. 2. Measure and record the temperature (should be around 10�C)

3. 3. Fill 3cm3 of glucose solution into the syringe and then in the same syringe insert 2 cm3 of the yeast solution

4. 4. Place the syringe into Beaker A (mouth facing up) and record the number of bubbles produced in 10, 15 and 20 minutes

5. 5. Fill Beaker B will 80 mL of water at room temperature (20�C)

6. 6. Fill 3cm3 of glucose solution into the syringe and then in the same syringe insert 2 cm3 of the yeast solution

7. 7. Place the syringe into Beaker B (mouth facing up) and record the number of bubbles produced in 10,15 and 20 minutes

8. 8. Set up a hotplate at 30C

9. 9. Fill Beaker C with 80 mL of water and place on hotplate

10.Fill 3cm3 of glucose solution into the syringe and then in the

same syringe insert 2 cm3 of the yeast solution

11.Place the syringe into Beaker C and record the number of

bubbles produced in 10,15 and 20 minutes

12.Repeat steps 9-11 for 40C and 50C in Beaker D and E

Data Analysis

From the data above it can be seen that the hypothesis was correct because the enzymatic activity did increase until the optimum temperature of 40C was reached and started to fall after that point.


In conclusion my hypothesis was correct because the rate or enzymatic activity did increase to a point and then dropped. This is due to the enzymes having an optimum temperature at which they catalyze reactions most efficiently. It can be seen that the optimum level for enzymatic activity is at 40 degrees Celsius because that is the peak of the curve and any point before or after that point has less enzymatic activity. At high temperatures the molecules, the enzymes and the substrates, move around fastest, and thus there are the most collisions per second, causing enzyme-substrate complexes to be formed more frequently and thus have a higher rate of reaction. Points after the optimum temperature result in a lower rate of reaction because the enzymes start to get denatured and lose enzyme structure and thus the substrate (e.g. glucose molecules) cannot bind to the active site of enzyme. So the higher the temperature the most enzymatic activity there will be until the optimum temperature and any temperature after that will result in denaturation of enzymes and drop in enzymatic activity.




The intervals between our temperatures was 10 degrees which is relatively inaccurate whereas we could have used a smaller gap between temperatures to gain more accuracy

Next time, I would use 2 degree gaps between my temperatures so I would have a more specific set of data and a smoother curve for the data.

The method in which we measured the enzymatic activity was very inaccurate as a result of human error because we manually counted the bubbles as we saw it. This was a very crude and inaccurate way of measuring because there were points were many bubbles came out at once so it was hard to count them all and there were many small ones attached to big ones so a few bubbles might have been counted twice or not counted at all.

Since there is not many ways in which this error can be improved, we could use another factor to measure enzymatic activity such as the pH level.

We can write a custom essay

According to Your Specific Requirements

Order an essay

You May Also Find These Documents Helpful

An Investigation of the Effect Enzyme Concentration...

Introduction This lab contains the use of a catalase which is an enzyme found in liver. The substrate used in this experiment is hydrogen peroxide. Enzymes are very sensitive organic molecules. They speed up chemical reactions. A high temperature, or change in pH could cause damage to an enzyme or affect its motion. In this lab the enzyme concentration is increased to see its effect...

Modifying a Simple Hydrolysis of Sucrose Experiment...

How can the Michaelis Menten constant for the dextrase content of yeast be calculated with simple experiment on hydrolysis of sucrose? Introduction: In anaerobic conditions yeast cells break down sugar molecules into ethanol and produce carbon dioxide. The process is called alcoholic fermentation. The equation of this process is: C6H12O6 -> 2 C2H5OH + 2 CO2 ↑+ E. The process consists of a series of...

Rate of Activity of the Enzyme Catalase...

In this study of increasing target temperature of enzyme catalase coated onto paper filter disks reacting through 3% hydrogen peroxide solution in culture tubes, there was an increase in amount of time taken for the enzyme catalase coated filter paper disks to float to the top of the 3% hydrogen peroxide solution in culture tubes (table 2) as the temperature of the catalase enzyme passed...

The Variation of Rate of Enzymatic Activity...

Introduction In this lab the rate of enzymatic activity will be tested by pouring hydrogen peroxide over cow liver. A reaction will take place its products are water and oxygen. The enzymatic activity rate will be compared between pieces of liver with different sizes, and the change in pressure in a bottle/container will be measured, which will indicate the enzymatic activity rate. Materials: * Device...

Investigation into the Enzyme Activity of Catalyze...

In this pre-designed procedure the enzyme activity of catalase in a potato was tested, in the form of measuring the volume of the Oxygen (O2) released by a catalase - hydrogen peroxide (H2O2) reaction in the span of one minute. The more oxygen released by the reaction the higher the enzyme activity was assumed to be, as more of the product was released in the...

Get Access To The Full Essay
Materials Daily
100,000+ Subjects
2000+ Topics
Free Plagiarism
All Materials
are Cataloged Well

Sorry, but copying text is forbidden on this website. If you need this or any other sample, we can send it to you via email.

By clicking "SEND", you agree to our terms of service and privacy policy. We'll occasionally send you account related and promo emails.
Sorry, but only registered users have full access

How about getting this access

Become a member

Your Answer Is Very Helpful For Us
Thank You A Lot!


Emma Taylor


Hi there!
Would you like to get such a paper?
How about getting a customized one?

Can't find What you were Looking for?

Get access to our huge, continuously updated knowledge base

The next update will be in:
14 : 59 : 59
Become a Member