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The Variation of Rate of Enzymatic Activity of Bos Primigenius Liver With Respect to Surface Area Essay Sample

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The Variation of Rate of Enzymatic Activity of Bos Primigenius Liver With Respect to Surface Area Essay Sample

Introduction

In this lab the rate of enzymatic activity will be tested by pouring hydrogen peroxide over cow liver. A reaction will take place its products are water and oxygen. The enzymatic activity rate will be compared between pieces of liver with different sizes, and the change in pressure in a bottle/container will be measured, which will indicate the enzymatic activity rate.

Materials:

* Device measuring pressure

* Liver

* Nalgene reaction bottle

* Syringe without a needle

* 3% solution of hydrogen peroxide

* Scalpel

* An electric scale

* Tweezers

* Computer with Logger Pro installed

Independent Variable:

* Mass of the liver piece

Dependent Variable:

* Change in pressure in the nalgene reaction bottle

Controlled Variables:

Variable controlled:

How?

Why?

Shape of the liver piece

Rectangular

The increase in mass will result from an increase in size, so that the surface area will be comparable, and from there the data as well

Amount of hydrogen peroxide poured into the container

10 mL

Being one of the reactors, the amount of hydrogen peroxide is a potential factor for the enzymatic activity rate, so it will be controlled

Amount of time of recording data

1 minute

In order to receive adequate data for processing, every trial should be run at the same conditions, including time, because pressure might keep on going to build up in the nalgene bottle

Procedure:

1. Set up the device for measuring the pressure

a. Assemble it

b. Plug it into the computer and open Logger Pro 3

c. Set up an “experiment” in the program for 60 seconds, data taken every second

2. Cut 12 pieces of liver

a. 3 pieces of liver with the mass of 1g, 1.5g, 2g and 2.5g

3. Put a piece of liver into the pyramid-like tube

4. Load the syringe with 15 mL of hydrogen peroxide

5. Press “start experiment” in LogoPro and pour the hydrogen peroxide into the nalgene reaction bottle and cover it with the device measuring pressure

6. Wait until the computer program finishes recording data

7. Save the data on the computer

8. Clean the tube

9. Repeat steps 3-8 with all of the liver pieces

Safety gear:

* Goggles

Data Collection and Processing

Graph #1. 1g cow liver pieces

X-axis – time (s). Y-axis – pressure (kPa)

In every trial, there was a drop in the beginning, followed by a steady raise in pressure. Trial #2 and #3 were very similar, even though #3 had a bigger drop in pressure in the beginning. Average ending pressure was 92.61 kPa.

Graph #2. 1.5g liver pieces

X-axis – time (s). Y-axis – pressure (kPa)

This time only the first trial had a drop in the beginning. The other two had a rapid increase in the beginning, with a smoother raise in pressure towards the end. The average ending pressure was 95.57.

Graph #3. 2g liver pieces

X-axis – time (s). Y-axis – pressure (kPa)

This data showed a great variety. All three trials are completely different from each other. During trial #3 the pressure reached was above 105 kPa. The average result though was 95.97 kPa.

Graph #4. 2.5g liver pieces

X-axis – time (s). Y-axis – pressure (kPa)

Trials #1 and #2 had a similar ending pressure, but the raise in pressure was different over time. Trial #3 was quite lower than the two before. The average ending pressure was 102.17 kPa.

Possible Errors:

* In some of the trials, when pouring the hydrogen peroxide (H2O2) into the nalgene bottle and taking the syringe out, there was a pressure drop. It was caused by not closing the hole, through which H2O2 was poured, before taking the syringe out.

* Liver pieces were not similar enough in shape. Some were thinner and longer, while other were thicker and shorter, resulting in different surface areas and therefore uncertainty in data.

* The measuring device was not of 100% precision and accuracy. It sometimes recorded the same amount of pressure for 2-4 seconds, and the starting pressure was not always the same.

* The speed of pouring in the H2O2 was not the same, resulting into some data having large spikes in the beginning of the trial

* Timing between trials of starting recording and pouring in the H2O2 was not same. This could have affected the data received, because the trials had different reaction times

Conclusion

A raise in pressure and difference in data between treatments proves that this method is viable and can be used for such types of experiments. The data suggests that an increase in surface area results in greater enzymatic activity. However, the amount of errors prevented a solid confirmation. These errors introduced uncertainties, which greatly affected the data.

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